Figure 7. Inhibition of Hh signaling during hMADS proliferation does not affect differentiation potential.

hMADS cells were grown in presence or in absence of cyclopamine (5 µM) until confluence for 10 days. Then cyclopamine was removed and cells were induced to differentiate into (A) adipocytes or (B) osteoblasts or (C) both (50% adipogenic and 50% osteogenic media). After 10 days mRNA were extracted and aP2, PPARγ, C/EBP-α, Runx-2, osteoprotegerin and ALPL expressions were evaluated by quantitative RT-PCR.