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. 2011 Feb 3;6(2):e16740. doi: 10.1371/journal.pone.0016740

Figure 4. Inhibition of TNFα production can reduce caspase activation and the number of annexin V-positive IPNV-infected cells.

Figure 4

(A) Detection of annexin V-positive cells following infection with IPNV. ZF4 cells were pre-treated with TNFα-specific siRNA or AG-126, infected with IPNV (MOI = 1), and incubated for 0, 6, 12, 18 and 24 h. Three individual experiments were performed for each sample. (B–D) Caspase-9, -8, and -3 activities were analyzed. ZF4 cells were pre-treated with TNFα-specific siRNA or AG-126 for 2 hours, infected with IPNV (MOI = 1), and incubated for 0, 6, 12, or 24 h. Luminogenic substrate assays were performed in triplicate. Data shown are the mean ± SD. Student's t tests indicate significant differences compared to IPNV infection only: *, p<0.05.