Table 1. Primer sequences for duplex siRNA that specifically targeted the mRNA encoding TNFα or RIP1 and scrambled siRNA.
Oligo Name | Sequence 5′→3′ | Oligo Name | Sequence 5′→3′ |
tnfa-siRNA-sense | CUCUGAAUCAAAGACCUUA | tnfa-siRNA- antisense | UAAGGUCUUUGAUUCAGAG |
rip1-siRNA-sense | CAAAUGCACUCAUUCCUGA | rip1-siRNA-antisense | UCAGGAAUGAGUGCAUUUG |
Scrambled-siRNA-sense | GAUCAUACGUGCGAUCAGA | Scrambled-siRNA-antisense | UCUGAUCGCACGUAUGAUC |