Table 2. Analysis of acid phosphatase secretion and effect of Rho3 overproduction.

	wt+vector	wt+rho3+OP	Δapm1+vector	Δapm1+rho3+OP
0 h	0±0	0±0	0±0	0±0
1 h	0.195±0.082	0.306±0.097	0.009±0.081	0.103±0.032
2 h	0.487±0.129	0.788±0.123	0.104±0.126	0.179±0.024
3 h	0.870±0.172	1.250±0.129	0.208±0.081	0.318±0.028
4 h	1.384±0.262	1.949±0.229	0.424±0.076	0.556±0.014
5 h	2.057±0.278	2.726±0.311	0.730±0.097	0.898±0.014

Wild-type cells and Δapm1 cells, which were transformed with either the pDB248 vector or the rho3⁺-containing vector, were assayed for acid phosphatase activity as indicated in the Materials and Methods section. Values from 3 independent experiments are expressed as means ± standard deviation.