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. 2011 Feb 1;17:341–354.

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Copyright © 2011 Molecular Vision.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Epithelial cells obtained from full thickness and superficial limbal explants cultured for 3 weeks in cholera toxin-free Green medium. Staining for CK3 (A: superficial limbal explants; B: full thickness limbal explants), vimentin (C: superficial limbal explants. D: full thickness limbal explants), broad spectrum cytokeratins (CK4, 5, 6, 8, 10, 13, and 18; E: superficial limbal explants; F: full thickness limbal explants), CK19 (G: superficial limbal explants; H: full thickness limbal explants), delta N p63α (I: superficial limbal explants; J: full thickness limbal explants), ABCG2 (K: superficial limbal explants; L: full thickness limbal explants) of limbal cells; M: negative control; N: positive control (human corneal epithelial cells stained with CK3). O: keratocytes stained with vimentin. Bars: 200 µm; magnification: 10×.