Table 1. Primers selected for PCR testing after reverse transcription.
Gene name | Sequence | PCR product size (bp) |
---|---|---|
Cytokeratin 3 |
F: 5′-CGTGGTCAGCAGCAGCACGA-3′ |
258 |
|
R: 5′-CGGTTGCTGGCCGAGCTGAA-3′ |
|
Vimentin |
F: 5′-TGGCCGACGCCATCAACACC-3′ |
257 |
|
R: 5′-CACCTCGACGCGGGCTTTGT-3′ |
|
Cytokeratin 19 |
F: 5′-GGTTGCTCCGTCCGTGCTCC-3′ |
270 |
|
R: 5′-TTCTCGTTGCCCGCCAGCAG-3′ |
|
ABCG2 |
F: 5′-AGTTCCATGGCACTGGCCATA-3′ |
395 |
|
R: 5′-TCAGGTAGGCAATTGTGAGG-3′ |
|
DeltaN p63α |
F: 5′-TGGCAAAATCCTGGAGCCAGAAGA-3′ |
104 |
|
R: 5′-GTGGCTCACTAAATTGAGTCTGGGC-3′ |
|
β-actin |
F: 5′-TCATGTTTGAGACCTTCAACACCC-3′ |
602 |
R: 5′-GTACTTGCGCTCAGGAGGAG-3′ |