Table 1. Primer sequences for quantitative RT–PCR.
Gene | Probe (5′-3′) | Forward primer (5′-3′) | Reverse primer (5′-3′) |
---|---|---|---|
ICAM-1 |
CCACGGAGCAGCACCACGGT |
GTGACCAGCCCAAGTTGT |
TCCCGTTTCAGCTCCTTCT |
occludin |
AAACCGCTTGTCATTCACTTTGCCA |
GGGACAAGGAACACATTTATGAT |
TGGATTTATAGGAAGACTCTGGAT |
18S rRNA | CGCCTGCTGCCTTCCTTGGATGTG | AGTCGCCGTGCCTACCAT | CGGGTCGGGAGTGGGTAAT |