FIG. 7.

The ΔSNAG construct acts as a dominant negative of Snail in E-cadherin promoter repression. (A) The activity of the E-cadherin promoter in Snail-expressing CarB cells was more efficiently derepressed by the ΔSNAG than by the ΔNt Snail mutant. (B) E-cadherin promoter activity in Snail-deficient PDV cells. The repression of the E-cadherin promoter induced by cotransfection of Snail was fully relieved by the ΔSNAG mutant but not affected by the ΔNt mutant. Promoter activity was determined 24 h after transfection, as indicated in the legend of Fig. 1.