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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Clin Cancer Res. 2010 Nov 22;17(3):472–482. doi: 10.1158/1078-0432.CCR-10-0568

Figure 4. HGF rescues CRC cells from cetuximab-induced G1 arrest, and PHA-665752 abrogates HGF rescue effect.

Figure 4

(A) DiFi, GEO, and LIM1215 cells were treated for 24 hours with complete media, cetuximab (5 nM), cetuximab +HGF (75 ng/ml), and cetuximab + HGF + PHA-665752 (0.4 uM) followed by flow cytometric determination of cell cycle distribution, as described in Materials and Methods. Cetuximab induced G1 arrest in all cell lines, which could be overcome by HGF. Addition of PHA-665752 abrogated the effect of HGF in cetuximab-treated cells. Representative results of three independent experiments.