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. Author manuscript; available in PMC: 2012 Feb 9.
Published in final edited form as: J Am Chem Soc. 2011 Jan 7;133(5):1459–1465. doi: 10.1021/ja108637s

Table 2.

Relative turnover rates for electrolysis and NADPH driven NNK oxidation in aerobic 50 mM phosphate buffer plus 0.1 M NaCl, pH 7.0.

Product Electrolysis (− 650 mV vs SCE) NADPH assay (see Methods)
P450 2E1/CPR+b5 Polyion/CPR+b5 Polyion/P450 2E1 P450 2E1/CPR+b5
HPB found (nmol) 0.24 ± 0.04 0.003 ± 0.001 0.03 ± 0.01 0.14 ± 0.03
Turnover rate h−1 (mol HPB)−1 (mol P450)−1 57 ± 9 ~ 0.7 7 ± 2.5 34 ± 6
H2O2 found (mM) 0.75 ± 0.12 0.73 ± 0.10 0.30 ± 0.06 -