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. Author manuscript; available in PMC: 2012 Feb 9.
Published in final edited form as: J Am Chem Soc. 2011 Jan 7;133(5):1270–1273. doi: 10.1021/ja109578b

Figure 2.

Figure 2

A. MoeO5 catalyzes the formation of 6 from 3PG (4) and FPP (5) via a mechanism proposed to involve isomerization to NPP (7). B. NPP forms during the MoeO5 reaction. NPP and FPP have the same [M-H]−1 (381.1237). LC/MS extracted ion chromatograms of a: an overlay of NPP and FPP standards (blue and red respectively) demonstrating small but reproducible differences in retention times; b: MoeO5 reaction of FPP spiked with NPP following quenching of the enzymatic reaction (green); c: MoeO5 reaction with FPP showing the formation of a new peak with a RT consistent with NPP (black).14