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. 2004 Jan;24(1):71–83. doi: 10.1128/MCB.24.1.71-83.2004

FIG. 10.

FIG. 10.

Hyperosmolarity promotes the endogenous interaction of Pak2 with Syk in B cells. (A) Ramos cells (20 × 106) were incubated in medium with or without 400 mM sorbitol. Cell lysates were immunoprecipitated (IP) with anti-Pak2 antibody or beads alone. The immunoprecipitated proteins were analyzed by immunoblotting with anti-Syk and anti-Pak2 antibodies. (B) The intracellular localization of endogenous Pak2 and Syk in B cells was analyzed immunohistochemically with anti-Pak2 and anti-Syk antibodies. The upper panels show the localization of Pak2 (left) and Syk (middle) in unstimulated cells and a merged image (right). The lower panels show the localization of the molecules in sorbitol-stimulated cells.