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Cdc42 regulates the association of Pak2 with Syk. COS-7 cells were transiently transfected with the cDNAs for WT HA-Pak2, Syk, HA-Cdc42L61, HA-Cdc42N17, and Flag-Cdc42L61, as indicated. At 48 h after transfection, cells were solubilized with lysis buffer and Pak2 was immunoprecipitated (IP) with anti-HA antibody. Immunoprecipitated proteins and detergent-soluble lysates were analyzed by immunoblotting with anti-pTyr, anti-Syk, anti-HA (Pak2 and Cdc42), and anti-Flag (Cdc42) antibodies.
