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Phosphorylation of Syk and Pak2 in vitro. GST-Syk (0.5 μg) was incubated with Pak2 and [γ-32P]ATP as described in Materials and Methods. (A) Samples were analyzed by SDS-PAGE, and the radiolabeled proteins were identified by autoradiography. (B) Phosphoamino acid analysis of Syk phosphorylated by Pak2. (C) Phosphoamino acid analysis of Pak2 phosphorylated by Syk. O, origin; pT, pY, and pS, phosphorylated threonine, tyrosine, and serine, respectively. The autoradiograms are shown.
