FIG. 8.

Pak2 and Syk cooperate in hyperosmolarity-induced activation of JNK. COS-7 cells were transfected with the combination of Syk, Pak2 (WT and K278R), and Cdc42 cDNAs as indicated (A) or Pak2, Cdc42L61, and the various PTK cDNAs were cotransfected into COS-7 cells (B). Cells were incubated in medium with or without the addition of 400 mM sorbitol. Total cell lysates were analyzed by immunoblotting with anti-phospho-JNK and anti-JNK antibodies. Positions of molecular mass markers are shown on the left in kilodaltons.