FIG. 5.

AGO1 cofractionates with GFP siRNAs. The salt-extracted material from the ribosome pellet of cells expressing BB2-tagged AGO1 and GFP dsRNA was loaded onto a Superdex 200 column, and selected fractions were subjected to Western blot analysis with anti-BB2 antibodies (A) and Northern blotted for the presence of GFP siRNAs (B). The elution positions of carbonic anhydrase (30 kDa), bovine serum albumin (66 kDa), and thyroglobulin (669 kDa) are indicated. A DNA size marker of 26 nt is indicated in panel B.