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. 2004 Jan;24(1):420–427. doi: 10.1128/MCB.24.1.420-427.2004

FIG. 6.

FIG. 6.

siRNA accumulation is dependent on AGO1. (A) The level of Ingi siRNAs was probed by Northern blotting in wild-type cells (wt; lane 1) and four independently cloned cell lines deficient in the AGO1 protein (ago1−/−; lanes 2 to 5). RNA size markers in nucleotides are indicated on the right. The hybridization to initiator methionyl tRNA (tRNA) served as a loading control. (B) Abundance of Ingi siRNAs in wild-type (lane 1), AGO1-deficient (lane 2), BB2-AGO1-complemented (lane 3), and TAP-AGO1-complemented (lane 4) cells. The level of 5S rRNA was used as a loading control. (C) Northern blot analysis for GFP siRNAs of RNA isolated from wild-type cells expressing GFP dsRNA (lane 1) and four independent clonal ago1−/− cell lines expressing GFP dsRNA (lanes 2 to 5). RNA size markers in nucleotides are indicated on the left. The hybridization to initiator methionyl tRNA (tRNA) served as a loading control. (D) Detection of GFP dsRNA in the cell lines described in panel C, with β-tubulin mRNA (β-tub) serving as a loading control.