Fig. 4.
Effect of the combination of ATP and ivermectin on calcium uptake by murine peritoneal macrophages. Macrophages from WT (upper panel) or P2X7-KO (lower panel) mice primed for 4 h with 250 ng/mL LPS were loaded with fura-2/AM. After washing, they were incubated in a medium without calcium or magnesium, in the absence or in the presence of 3 μM ivermectin (IVER). Ninety seconds, 2 and 5 min after the start of the measurement, 100 μM EGTA, 10 μM ATP, and 1 mM CaCl2 were successively added to the cuvette. Results are the means + SEM of three experiments