Fig. 8.

Effect of the combination of ATP plus ivermectin on the release of IL-1β from murine peritoneal macrophages. Upper panel Macrophages from WT mice primed for 4 h with 250 ng/mL LPS were incubated for 15 min at 37°C in the presence of 1 mM CaCl₂ and in the absence of magnesium, in the presence of increasing concentrations of ATP or of 10 μM nigericin (NIG). Lower panel Macrophages from WT or P2X₇-KO mice primed for 4 h with 250 ng/mL LPS were incubated for 15 min at 37°C in the presence of 1 mM CaCl₂ and in the absence of magnesium, in regular HBS medium or in a medium with high potassium (100 mEq/L). The cells were incubated in the absence (open bars) or in the presence (squared bars) of 10 μM ATP and in the absence or in the presence of 3 μM ivermectin (IVER). The IL-1β content of the supernatant was assayed by ELISA after centrifugation and sonication. Results are the means + SEM of six experiments. **p < 0.01 when compared to control