FIG. 4.

Confirmation of the interaction between N2N and NE. (A) N2N directly binds to NE in GST pull-down assays. (B) Cotransfected N2N and NE form an immunoprecipitable complex in HeLa cells. The arrow indicates the NE bands, and the star indicates the immunoglobulin G bands. IP, immunoprecipitation; WB, Western blot; α-Myc, anti-Myc antibody. (C) Endogenous N2N coprecipitates with transfected NE from HeLa cells. (D) The unique C24 domain of N2N is required but not sufficient for interaction with NE in the yeast CytoTrap two-hybrid assay. Three independent clones are shown for each interaction.