FIG. 5.

Cleavage of N2N by NE. (A) Recombinant GST-N2N fusion proteins cleaved by NE in vitro, Coomassie-stained SDS-polyacrylamide gel. Note that GST itself and the unique C24 domain of N2N are not cleaved by NE. (B) Representative Western blot result of endogenous Notch2 and N2N digested by NE in U937 cells when NE is added to the culture medium. (C) A representative autoradiogram of immunoprecipitation of endogenous Notch2 and N2N digested by NE in ³⁵S-labeled U937 cells when NE is added to the culture medium.