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. 2011 Feb;13(2):98–107. doi: 10.1593/neo.101088

Figure 1.

Figure 1

Figure 1

H-Ras HVR determines the invasive/migratory phenotypes of MCF10A cells. (A) Sequence alignment of H-Ras/N-Ras HVR. (B) Schematic outline of chimera. All of the Ras constructs contain an oncogenic mutation at codon 12 (G12→D). (C, upper) Cell lysates were collected, and Ras expression was detected by immunoblot analysis. β-Actin was used as a loading control. H-Ras activity (H-Ras-GTP) was measured as described in Materials and Methods. (C, lower) RT-PCR analysis was performed. (D) Cells were subjected to in vitro invasion assay and Transwell migration assay. The number of invaded or migrated cells per field was counted (x400) in 13 arbitrary visual fields. The results represent means ± SE of triplicates. *Statistically different at P < .01, by 2-tailed Student t test.