Figure 3.

H-Ras HVR are necessary for MMP-2 up-regulation. (A, B) Conditioned media were collected and analyzed for expression of MMP-2 and MMP-9 by gelatin zymogram assay (upper) and immunoblot analysis (lower). (C) Knockdown of MMP-2 by siRNA was confirmed by gelatin zymography and immunoblot analysis in Nh166–189 cells. Control cells were treated with Stealth RNAi Negative Control Duplexes. (D) Cells transfected with siRNA targeting MMP-2 was subjected to an in vitro invasion assay (left panel) or a Transwell migration assay (right panel). The number of invaded or migrated cells per field was counted (x400) in 13 arbitrary visual fields. The results represent means ± SE of triplicates. *Statistically different from control at P < .01.