Skip to main content
. 2011 Feb;13(2):98–107. doi: 10.1593/neo.101088

Figure 4.

Figure 4

Pro173 and Pro174 are important for H-Ras-induced invasive/migratory phenotypes. (A) Schematic outline of proline mutants of H-Ras. (B) H-Ras activity (H-Ras-GTP) was measured as described in Materials and Methods. Band intensities were quantitated and plotted. **Statistically different at P < .01. (C) Rac-1 activity (Rac-1-GTP) was measured as described in Materials and Methods. Band intensities were quantitated and plotted. **Statistically different at P < .01. (D) Cells were subjected to the in vitro invasion assay and Transwell migration assay. The number of invaded or migrated cells per field was counted (x400) in 13 arbitrary visual fields. The results represent means ± SE of triplicates. *,**Statistically different from control at P < .05 and P < .01, respectively, by the 2-tailed Student t test.