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. 2004 Jan;24(1):164–171. doi: 10.1128/MCB.24.1.164-171.2003

FIG. 10.

FIG. 10.

Bcl10-CARMA1(ΔCD) fusion protein rescues the functional defect in JPM50.6 cells. (A) JPM50.6 cells were transfected with plasmids encoding a NF-κB-dependent luciferase gene in the presence of expression constructs encoding Bcl10, CARMA1(ΔCD), Bcl10-CARMA1(ΔCD), CARMA1, or the vector control. The transfected cells were stimulated with (i) medium, (ii) 1 μg (each) of anti-CD3 and anti-CD28 antibodies per ml, or (iii) 10 ng of PMA per ml and 1 μg of anti-CD28 antibodies per ml for 6 h. The cells were lysed, and luciferase activity was determined as described in the legend to Fig. 1. Unstim, unstimulated. (B) The expression of transfected constructs was examined by Western blotting (WB) with anti-Myc or anti-Bcl10 antibodies.