FIG. 6.

PKC-θ forms a complex with CARMA1. (A) HEK293 cells were transfected with plasmids encoding Myc-tagged PKC-θ together with Flag-tagged CARMA1, Flag-tagged RIP, Flag-tagged Vav, and the vector control (A) or Flag-tagged CARMA1 and its deletion mutants (B). The transfected cells were lysed and immunoprecipitated (IP) with anti-Flag antibody-conjugated agarose. The resulting immunoprecipitates were subjected to SDS-PAGE and analyzed by Western blotting (immunoblotting [IB]) with antibodies specific for Myc tag (top panel) or Flag tag (bottom panel).