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. 2004 Jan;24(1):398–406. doi: 10.1128/MCB.24.1.398-406.2004

FIG. 1.

FIG. 1.

Analysis of S. pombe chromosomal rDNA replication by 2D agarose gel electrophoresis. The upper diagram represents an rDNA repeat unit, where the regions coding for the mature rRNAs (black boxes), the NTS, and the 17-bp reb1p binding sequences (striped boxes) are indicated. Restriction site abbreviations: B, BamHI; BII, BglII; H, HindIII; K, KpnI; S, SalI; X, XhoI. Horizontal lines labeled A through E represent the restriction fragments analyzed in the panels below. The arrow in panel A points to the elongated signal of accumulated replication intermediates. Arrowheads in panel B indicate three spots of accumulated intermediates labeled 1, 2, and 3, which correspond to barriers RFB1, RFB2, and RFB3, respectively. The open arrowhead in panel C points to the signal corresponding to replication intermediates containing a growing bubble. The probe used for 2D gels in panels A, B, and C was the 3.0-kb HindIII-KpnI fragment spanning most of the NTS and the 5′ end of the transcription unit. The probe used for 2D gels in panels D and E was the 3.5-kb SalI-BglII fragment within the coding region.