Table II. Summary of peptide data for best transitions studied.
| Protein peptide sequence | Precursor ion m/z | Fragment ion m/z | Standard interface |
Dual ion funnel interface |
|||
|---|---|---|---|---|---|---|---|
| LOD | CV | LOD | CV | CV at thermo LODa | |||
| ng/ml | % | ng/ml | % | % | |||
| Bovine carbonic anhydrase | |||||||
| VLDALDSIK | 487.28192+ | 874.4880+ (y8) | 400 | 20.4 | 80 | 23.5 | 9.7 |
| DFPIANGER | 509.75132+ | 378.7036+2 (y72+) | 80 | 38.3 | 40 | 36.6 | 11.6 |
| E. coli β-galactosidase | |||||||
| VDEDQPFPAVPK | 671.33792+ | 755.4450+ (y7) | 400 | 45.3 | 80 | 24.3 | 18.2 |
| LWSAEIPNLYR | 681.36422+ | 662.3620+ (y5) | 4,000b | 16.4 | 4,000b | 5.5 | 5.5 |
| Equine skeletal muscle myoglobin | |||||||
| LFTGHPETLEK | 424.55923+ | 506.25892+ (y92+) | 80 | 25.2 | 80b | 5.3 | 5.3 |
| YLEFISDAIIHVLHSK | 629.01213+ | 740.41962+ (y132+) | 40,000 | 9.9 | 4,000 | 4.2 | 10.2 |
| Chicken ovalbumin | |||||||
| HIATNAVLFFGR | 673.37242+ | 1,095.5946+ (y10) | 40,000b | 40.0 | 40,000b | 2.5 | 2.5 |
| GGLEPINFQTAADQAR | 844.42363+ | 666.33882+ (y122+) | 4,000 | 13.0 | 80 | 14.9 | 7.24 |
| Bovine cytochrome c | |||||||
| EDLIAYLK | 482.77112+ | 494.2973+ (y4) | 400 | 54.4 | 80 | 7.6 | 3.5 |
| TGQAPGFSYTDANK | 728.83882+ | 1,099.5055+ (y10) | 400 | 79.1 | 40 | 9.4 | 11.9 |
a Percent CV for selected best transitions at the concentration of the LOD for the standard Thermo interface obtained with the dual ion funnel interface.
b Interference peaks from the matrix were observed to limit the detection and quantification of the peaks of interest.