Table I. Eight representative substrate kinase motifs matched to phosphopeptides identified in this study.
A summary of eight representative substrate kinase motifs predicted to be matched to the identified phosphopeptides is shown. A total of 29 identified phosphopeptides matched the consensus substrate motifs of these eight kinases. The name of each kinase and the name of the identified phosphoproteins (Kinase and phosphoprotein), the experimentally identified phosphopeptide sequence (Phosphopeptide sequence), function of the phosphoproteins (Function), peptide fragmentation methods (MS/MS), and the references of previously reported phosphorylation sites (Refs.) are listed. A more comprehensive overview is provided in supplemental Table S4 with 29 consensus matches. EGFR, EGF receptor. FYVE, Fablp, YoTB, Vaclp, and EEA domain-containing proteins. Lowercase letters in sequences indicate modified residues.
| Kinase and phosphoprotein | Phosphopeptide sequence | Function | MS/MS | Refs. |
|---|---|---|---|---|
| Pyruvate dehydrogenase kinase | ||||
| Pyruvate dehydrogenase E1-α | YHGHsMSDPGVSYR | TCA cycle | ETD | 16, 40 |
| Ubiquinol-cytochrome c reductase core protein 1 | RLsRTDLTDYLNR | ETC complex III | ETD | |
| Netrin receptor UNC5B | ICsSLDAPNSRGNDWR | Apoptosis | ETD | |
| Ras-related protein Rab-3B | MAsVtDGKTGIK | Transport | ETD | |
| Zinc finger protein 811 | EILRNVVsIGDIRK | Transcription | ETD | |
| Microtubule-associated protein 6 | AsGADERDTRR | Binding | ETD | |
| Hypothetical ATP/GTP-binding site motif A (P-loop)-containing protein | RQGsDVDVLK | Binding | ETD | |
| Src kinase | ||||
| Pyruvate dehydrogenase E1-α | KGGCAKGKGGSMHMyGK | TCA cycle | ETD | |
| Pantothenate kinase 1 | LVKDIyGGDyER | Biosynthesis | ETD | |
| Cytosol aminopeptidase | mtKGLVLGIyAKDK | Proteolysis | ETD | |
| Arginase-2, mitochondrial | NyDIQYFSMR | Urea cycle | ETD | |
| Methylmalonyl-CoA mutase | GyDSDNPRVRGDVG | TCA cycle | CID | |
| Zymogen granule membrane protein 16 | TSSYSGEyGGKGGKR | Other | ETD | |
| Src family kinase | ||||
| Retinaldehyde-binding protein 1 | LQyPELFDsLSMEALR | Transport | ETD | |
| Transmembrane channel-like protein 2 | LTVSDmLVTyLTILVGDFL | Other | CID | |
| Branched-chain α-ketoacid dehydrogenase | ||||
| 2-Oxoisovalerate dehydrogenase subunit α, mitochondrial | IGHHSTSDDSSAYRsVDEVNYWDKQDHPISR | Metabolism | ETD | 16, 40, 45, 46 |
| GSK3, ERK1, ERK2, CDK5 | ||||
| Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex | EGPGAPCGsPR | TCA cycle | ETD | |
| Apolipoprotein B100 receptor | AEGSItPmETEGLLR | Metabolism, transport | ETD | |
| 40 S ribosomal protein S20 | RLIDLHsPSEIVK | RNA binding | ETD | |
| Junctophilin-2 | GLGTGLPERPREsPQLHER | Protein binding | ETD | |
| FYVE and coiled coil domain-containing protein 1 | AEKGEKtPPDTELHQEPVPADLVLK | Transport | ETD | 46 |
| Bcl-2-like 13 protein | AEKTsPTPSVFVELGEEELEAVTARPEAVER | Apoptosis | ETD | 16 |
| Disks large-associated protein 5 | ELGNIHETSQDLsPEK | Signaling | ETD | 47, 48 |
| Coiled coil-helix-coiled coil-helix domain-containing protein 2 | RAPAAQPPAAAAPSAVGsPAAAPR | Biosynthesis | ETD | 16 |
| PKCϵ kinase | ||||
| Zinc finger, CCHC domain-containing 5 | EPREPsAIsELR | Binding | ETD | |
| Cardiac phospholamban | RAsTIEMPQQAR | Transport | CID | 13, 49 |
| EGFR kinase | ||||
| Pantothenate kinase 1 | LVKDIyGGDyER | Biosynthesis | ETD | |
| Zymogen granule membrane protein 16 | TSSYSGEyGGKGGKR | Exocytosis | ETD | |
| GSK3 kinase | ||||
| Regulating synaptic membrane exocytosis protein 2 | sMQRsQSR | Exocytosis | ETD |