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Plasma membrane purification. A, experimental work flow outlining purification of Ad and nAd membranes from MDCK and 21D1 cells. Membrane protein isolation and purification are based on the cationic colloidal silica method (26) and a modified version (31). B, SDS-PAGE protein gel banding patterns of Ad and nAd isolated membrane fractions. Gel lanes were excised and trypsinized, and extracted peptides were subjected to LC-MS/MS protein identification.
