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LC-MS setup schematics (left) and total ion chromatogram (right) for on-line digestion of myoglobin. Insets show mass spectra extracted at the indicated LC elution times. a, control experiment (no digestion). b, on-line digestion. b.1 and b.2, effect of the insertion of the delay/trapping capillary without (b.1) and with (b.2) pepsin infusion.
