Fig. 5.

BmTXKβ-related toxins. A, multiple sequence alignment. Identical residues are shadowed in yellow, and conservative replacements are in red for acidic residues, green for hydrophobic residues, and blue for basic residues. NHD(L), long N-terminal helical domain; NHD(S), short N-terminal helical domain; CCD, C-terminal CSαβ domain. B, phylogeny. C, overall folding of MeuTXKβ5 short N-terminal helical domain. D, electrostatic potential map of the MeuTXKβ5 C-terminal CSαβ domain whose structure was built based on scyllatoxin (Protein Data Bank code 1SCY). E, structural superimposition of the MeuTXKβ5 C-terminal CSαβ domain and ChTX with the dyad shown in ball-stick models.