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Assay of haploid invasive growth. Strains MCY4702 (wild type) and MCY4622 (gal83Δ), expressing Gal83, Gal83W184A,R214Q, or Gal83G235R were assayed for invasive growth. Photographs were taken after 2 days and are representative of data from three experiments. Invasion of agar by cells with Gal83 was evident after 3 days. Plasmids were pRS313, pRT12, pHW39, and pOV81.
