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. 2010 Apr 21;10(2):M900521-MCP200. doi: 10.1074/mcp.M900521-MCP200

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 4. — Separation and isolation of fractions containing antigenic targets for MAb-D9-F10 clone. To further characterize and isolate antigenic targets, B. xylophilus protein extracts were fractionated by anion exchange chromatography. In total, 101 fractions were recovered and tested by performing Western blot analysis with MAb-D9-F10. Fraction numbers 27 to 43 contained an antigenic target for MAb-D9-F10. The arrow indicates the MAb-D9-F10-specific bands. The line indicates the increasing linear gradient of NaCl. The y axis indicates relative protein concentration.