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. 2010 Nov 3;10(2):780–789. doi: 10.1021/pr100927x

Figure 1.

Figure 1

UV chromatograms of proteolyzed cross-linked antibodies analyzed using a monolithic column. αRAD21 (panels (a) and (b)) and αAPC3 (panels (c) and (d)) antibodies cross-linked to AffiPrep A beads were incubated with trypsin and LysC, respectively. At the indicated time points an aliquot, which corresponded to 2 pmol antibody (assuming complete cleavage) was separated using a monolithic column. Increasing absorption in the UV chromatograms indicate proteolytic cleavage of the antibodies. For better illustration, chromatograms are displayed with a 5% time and a 15% signal offset.