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Coexpression of M3R and Tmem147 transcripts in several mouse tissues. Total RNA was prepared from the indicated mouse tissues (C57BL/6NTac mice). RT-PCR studies were carried out as described under Materials and Methods using gene-specific primer pairs (M3R, top; Tmem147, bottom). The sizes of the PCR products were the following: M3R, 158 bp; and Tmem147, 156 bp. RT, reverse transcriptase; C. cortex, cerebral cortex; Subm. gland, submandibular gland.
