Fig. 2.

Carbachol-induced yeast growth mediated by activation of WT-M3 or M3-Cub receptors. The indicated receptors were expressed in the MPY578q5 yeast strain expressing a hybrid chimeric yeast/mammalian G protein α subunit (for details, see Materials and Methods). Yeast growth was measured by determining the absorbance at 630 nm, either in the absence or presence of increasing concentrations of the muscarinic agonist, carbachol. The curves shown are representative of three independent experiments, each carried out in triplicate. Data are given as means ± S.E.M.