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. 2011 Feb;79(2):251–261. doi: 10.1124/mol.110.067363

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Fig. 5. — Tmem147 is found in the ER of transfected COS-7 cells. The subcellular localization of Tmem147 was studied via confocal microscopy using COS-7 cells transiently expressing Tmem147-FLAG. A, Tmem147 is localized to the ER (pDsRed2-ER represents an ER-specific marker) (Free et al., 2007). B and C, Tmem147 is not present in the Golgi (GM130 is a Golgi marker protein) or in early endosomes (EEA1 is a marker protein for early endosomes). All studies were carried out with cells permeabilized with 0.2% saponin. To visualize Tmem147-FLAG, Alexa-594- (red), or Alexa-488-conjugated (green) secondary antibodies were used in B and C, respectively (after incubation with a polyclonal anti-FLAG antibody). For experimental details, see Materials and Methods.