Fig. 7.
Effect of Tmem147 on M3R expression levels and M3R-mediated calcium responses in cotransfected COS-7 cells. A, effect of Tmem147 expression on M3R receptor expression levels (Bmax), as determined in [3H]NMS saturation binding assays. B, effect of Tmem147 expression on M3R-mediated calcium responses. Carbachol-induced increases in intracellular calcium levels were measured by using FLIPR technology. All experiments were carried out using COS-7 cells that had been cotransfected with plasmids coding for the human M3R and Tmem147 (see Materials and Methods for details). Data are presented as means ± S.E.M. of three independent experiments carried out in duplicate. RFU, relative fluorescence units.