Fig. 4.

The effect of T. b. brucei infection on the R_Tissue values for [³H]nifurtimox (a) and [¹⁴C]sucrose (b) in the brain regions. BALB/c mice were infected intraperitoneally with 2 × 10⁴ trypanosomes and in situ-perfused on days 7, 21, 28, and 35 p.i. and compared with noninfected control mice. All data points represent the mean ± S.E.M. (n = 4–5 for each infected group and n = 9 for noninfected control). The difference in the mean values for [¹⁴C]sucrose among the different postinfection groups was greater than would be expected by chance after allowing for the effects of differences in brain region (*P ≤ 0.001; two-way ANOVA). A Tukey's multiple comparison test indicated that overall the day 35 p.i. group was significantly different from the noninfected control group (P < 0.001), day 7 p.i. group (P < 0.001), day 21 p.i. group (P < 0.001), and day 28 p.i. group (P < 0.001). The significant variation in the overall distribution of [¹⁴C]sucrose to the different brain regions after allowing for the effects of infection was as reported for the multiple time uptake data (P ≤ 0.001; two-way ANOVA). Overall, there was no significant difference in the mean values for [³H]nifurtimox among the different infection groups after allowing for the effects of differences in brain region (P < 0.097). In contrast, there was difference for [³H]nifurtimox (P = 0.001) distribution to the different brain regions, and the pairwise multiple comparison procedure indicated that [³H]nifurtimox distribution to the thalamus was significantly larger than that found in the caudate putamen (P < 0.003), frontal cortex (P < 0.031), and hippocampus (P < 0.043), but not significantly different from any of the other brain regions. Data are not shown for caudate putamen and hippocampus.