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The pH profile of MBP-D30vST3Gal-I-His6. Activity was measured at indicated pH at 37°C for 30 min. Buffers (200 mM) used: sodium acetate (pH 4.5), MES (pH 5.0–6.5), HEPES (pH 7.0–7.5), Tris–HCl (pH 8.0–9.0), 3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acid (pH 9.5–11.0).
