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Schematic illustration for the one-pot two-enzyme preparative scale synthesis of sialyl LexβProN3 Neu5Acα2–3Galβ1–4(Fucα1–3)GlcNAcβProN3 from Neu5Ac, CTP and LexβProN3 Galβ1–4(Fucα1–3)GlcNAcβProN3. Enzymes used: MBP-Δ30vST3Gal-I-His6 and a recombinant NmCSS.
