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Apparent kinetic parameters of recombinant MBP-Δ30vST3Gal-I-His6 and PmST1
| Enzyme | Substrate | Km (mM) | kcat (min−1) | kcat/Km (mM−1 min−1) |
|---|---|---|---|---|
| MBP-Δ30vST3Gal-I-His6 | CMP-Neu5Ac | 1.4 ± 0.2 | 32 ± 2 | 23 |
| LacβMU | 1.2 ± 0.1 | 33 ± 1 | 28 | |
| LexβMU | 3.3 ± 0.5 | 4.7 ± 0.2 | 1.5 | |
| PmST1 | CMP-Neu5Aca | 0.44 | 1.9 × 103 | 4.4 × 103 |
| LacβMUa | 1.4 | 2.8 × 103 | 2.0 × 103 | |
| LexβMU | 18 ± 2 | (4.0 ± 0.2) × 102 | 23 |
