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. Author manuscript; available in PMC: 2011 Feb 4.
Published in final edited form as: Mol Immunol. 2008 Oct 17;46(4):677–687. doi: 10.1016/j.molimm.2008.08.281

Figure 3.

Figure 3

Inhibition of GSK3β with specific siRNA suppresses TNF-α and enhances IL-10 by F. tularensis-stimulated macrophages. (A) Raw 264.7 cells were pretreated with medium, control siRNA or siRNA specific for GSK3β for 96 h and the level of total GSK3β and p38 in the whole cell lysates were analyzed by Western blot. (B) Untransfected Raw 264.7 cells or cells transfected with control or specific siRNA were stimulated with F. tularensis LVS (MOI = 10) for 24 h, and the level of TNF-α and IL-10 in the culture supernatants were analyzed by ELISA.