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The role of IFN-γ on F. tularensis LVS-induced GSK3β (A) and NF-κB (B) activity. Macrophages from C57BL/6 wt mice were untreated or pretreated with IFN-γ (200 U/ml) overnight before stimulation with F. tularensis LVS (MOI = 10) for 30 or 60 min. Equal amounts of whole cell lysates or nuclear extracts were analyzed for GSK3β phosphorylation (A) or NF-κB p65 activation (B) using Western blot or TransAM assay, respectively. Data are representative three separate experiments.
