FIG. 1.
Constructions of recombinant MV-envHIV89.6. (A) Full-length gp160 and secreted gp140 HIV-1 Env proteins. Shaded boxes indicate V1, V2, and V3 regions; dashed boxes show the transmembrane domain. Amino acid positions are indicated. The BbsI and MfeI restriction sites used to introduce the ΔV3-AAELDKWASAA mutation are also indicated. (B) pMV(+) vectors with ATU containing a green fluorescent protein (GFP) gene in positions 2 and 3. The MV genes are indicated as follows: N, nucleoprotein; PVC, phosphoprotein and V C proteins; M, matrix; F, fusion; H, hemagglutinin; and L, polymerase. T7, T7 RNA polymerase promoter; T7t, T7 RNA polymerase terminator; ∂, hepatitis delta virus ribozyme.