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(A) Plasmid DNA profiles of Klebsiella isolates from patient 1 (Kpn1014, Kpn1016, and Kox1015) and patient 2 (Kpn1017) resolved on a 0.6% agarose gel. E. coli V517 supercoiled plasmid standards (kb). C, chromosomal DNA. (B) Southern blot of the gel shown in panel A after hybridization with an 1009-bp blaKPC-2-specific probe. DNA sequence-confirmed blaKPC-2 PCR product (right-most lane) loaded one hour before completion of the electrophoresis served as the positive control.
