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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Virus Res. 2010 Oct 8;155(1):181–188. doi: 10.1016/j.virusres.2010.10.001

Figure 4. Crosslink analysis of NC proteins.

Figure 4

WT (A-C, G-I) and HA (D-F, J-L) virus samples were mock-treated (A, D, G, J) or crosslinked with 0.1 mM (B, E, H, K) or 1 mM (C, F, I, L) BMH. After crosslinking, protein samples were separated by SDS-PAGE on a 16% acrylamide gel, and proteins were detected by immunoblotting with primary anti-NC antibodies (A-F) or a primary anti-HA antibody (G-L). Marker proteins were separated in lane M, and their sizes are indicated on the right hand side. Also indicated are the migration positions of the PrGag proteins, the WT monomer (NC) and dimer ([NC]2) proteins, and the HA nucleocapsid monomer (HANC) and dimer ([HANC]2) proteins.