FIG. 3.

Southern blot analysis of genomic DNA from HeLa and A549 cells after cotransduction with HC-AdFLPe (20 MOI) and the indicated substrate vectors (20 MOI each) at early (a) and late (b) time points posttransduction. Signals at 2,200 bp indicate recombined, circular substrate vectors; signals at 3,300 bp indicate unrecombined, linear substrate vectors. HC-AdFLPe and the cellular HPRT fragment are represented by the signals at 6,800 bp. +, selection with hygromycin. Selection was terminated at day 50 posttransduction (−). Ctr, untransduced control cells.