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(a) Time course of EGFP expression in HeLa cells after cotransduction with HC-AdFLPe (100 MOI) and HC-AdSVEBNAs or HC-AdSVEBNAsΔFR (100 MOI each), respectively. Cells were analyzed for EGFP-specific fluorescence by flow cytometry. (b) Southern blot analysis of genomic DNA from the same HeLa cells. The circular substrate vector genomes are indicated by signals at 2,200 bp; the linear substrate vector genomes are indicated by signals at 3,300 bp. Ctr, untransduced control cells.
